Fig. 4

USP13 removed the K29- and K48-linked polyubiquitination chain of WWP1. A WB results of WWP1 expression upon USP13 knockdown in HCC cells. B WB results of WWP1 expression upon USP13 overexpression in HCC cells. C, D The ubiquitination of WWP1 was analyzed in HCC cells with stable USP13 knockdown or overexpression. E WWP1 protein level was detected in HCC cells treated with CHX and MG132 or HCQ. F CHX assays were conducted to detect the half-life of WWP1 in Huh7 shUSP13 and shNC cells. G Quantitative analysis of WWP1 protein expression by ImageJ. H, I WB results of the ubiquitination of WWP1 in HEK293T cells with co-transfection of Myc-WWP1, HA-ubiquitin, or mutations, and either the empty vectors or Flag-USP13